A Comprehensive Review on Tulsi and Guava Based Herbal Mouthwash

The present study focuses on the formulation and evaluation of a herbal mouthwash prepared from Tulsi (Ocimum sanctum) and Guava (Psidium guajava) leaves, both of which are rich in phytochemicals known for their antimicrobial, anti-inflammatory, antioxidant, and wound- healing properties. Conventional chemical mouthwashes, though effective, often cause undesirable effects such as tooth staining, mucosal irritation, altered taste, and dryness. This has increased the demand for safe, natural, and biocompatible herbal alternatives. In this study, fresh Tulsi and Guava leaves were collected, shade-dried, powdered, and extracted using the Soxhlet method with hydroalcoholic solvent. The obtained extracts were incorporated into a mouthwash formulation and evaluated for organoleptic properties, pH, foaming capacity, viscosity, stability, and antimicrobial activity. Phytochemical screening confirmed the presence of tannins, flavonoids, terpenoids, and essential oils responsible for therapeutic action. The prepared herbal mouthwash showed satisfactory clarity, acceptable pH (5.5–6.5), good stability, and significant antimicrobial activity against oral pathogens such as Streptococcus mutans, indicating its potential efficacy in reducing plaque, gingivitis, and oral infections.The results support that a Tulsi–Guava–based herbal mouthwash can serve as a safe, economical, and effective alternative to commercial chemical rinses. Its natural bioactive constituents not only inhibit microbial growth but also promote oral tissue healing, making it a promising formulation for long-term oral hygiene maintenance.