New validated reference genes for gene expression studies in the differentiating human airway epithelium
Abstract
The aim of the study was to identify stably expressed genes suitable for RT-qPCR normalization in an in vitro model of human airway epithelium (AE). The most stably expressed genes were identified using RNAseq and microarray data from AE cultures representing various stages of cell differentiation. Experimental validation of the top 15 genes with various expression levels was performed using RT-qPCR and several algorithms available in RefFinder tool.
Fields of Study & Subjects
Molecular Biology Techniques and Applications
Gene expression and cancer classification
RNA Research and Splicing
Publication details
- Journal / source
- Zenodo (CERN European Organization for Nuclear Research)
- Publication date
- 12 February 2026
- Language
- EN
- Document type
- Dataset