CRISPR-Cas-assisted transformation of Botrytis cinerea

Zenodo (CERN European Organization for Nuclear Research) 2026 Book chapter Open access

Read full text
Authors: Nassim Safari, Matthias Hahn
DOI: 10.5281/zenodo.18625813
Cited by: 1

Abstract

This chapter provides an overview of CRISPR-Cas-mediated strategies for Botrytis cinerea genome editing, which is followed by a detailed, step-for-step protocol for B. cinerea protoplast-mediated transformation with Cas9-gRNA ribonucleoprotein complexes and appropriate repair templates if required.

Fields of Study & Subjects

CRISPR and Genetic Engineering Fungal Plant Pathogen Control Fungal and yeast genetics research

Publication details

Journal / source
Zenodo (CERN European Organization for Nuclear Research)
Publication date
13 February 2026
Language
EN
Document type
Book chapter