USN Universal Barcode‑Ready PCR2 System for Ion Torrent
Abstract
This protocol describes a two-step PCR barcoding workflow developed at the University of South-Eastern Norway. PCR1 uses locus-specific primers with universal overhangs, and PCR2 introduces custom USN-designed barcodes and full Ion Torrent adapters. The method provides a flexible, modular, and contamination-aware approach for preparing sequencing-ready amplicon libraries.
Fields of Study & Subjects
Genomics and Phylogenetic Studies
Environmental DNA in Biodiversity Studies
Molecular Biology Techniques and Applications
Publication details
- Journal / source
- Zenodo (CERN European Organization for Nuclear Research)
- Publication date
- 13 February 2026
- Language
- EN