Type-I Interferon drives T-cell responses to amyloid-beta in the central nervous system

This repository contains raw and processed targeted spatial transcriptomics data generated from cerebral cortex sections of APP23^het transgenic and wild-type mice at 13 and 24 months of age, as well as human Alzheimer’s disease cortical tissue. Spatial profiling was performed using the Molecular Cartography™ platform (Resolve Biosciences), an imaging-based highly multiplexed single-molecule fluorescence in situ hybridization (smFISH) technology. The dataset includes raw transcript coordinate files, as well as cell segmentation outputs generated by the platform’s segmentation algorithm. Both segmentation-free (transcript-based) and segmentation-based spatial analyses were performed. These data were used to investigate age-dependent immune cell composition and spatial enrichment of T-cell phenotypes in amyloid plaque-associated regions, as described in the associated manuscript. Detailed experimental procedures, gene panels, preprocessing steps, normalization, clustering, and spatial analysis methods are provided in the Methods section of the publication.