Multivariate UV Optimization of Topiroxostat in Pharmaceutical Dosage Form

A simple, accurate, precise, and cost-effective multivariate UV spectrophotometric method was developed and validated for the quantitative estimation of Topiroxostat in bulk drug and pharmaceutical tablet dosage form. The method is based on multivariate calibration using absorbance measurements at five selected wavelengths (271, 273, 275, 277, and 279 nm) using methanol as the solvent. Calibration curves were linear in the concentration range of 5–25 µg/mL with good correlation coefficients (R² > 0.99) at all selected wavelengths. The developed method was validated as per ICH Q2(R1) guidelines for parameters including linearity, accuracy, precision, sensitivity, and robustness. The accuracy of the method was confirmed by recovery studies at different concentration levels, showing satisfactory percentage recovery. Precision studies demonstrated low %RSD values, indicating good repeatability and reproducibility. The assay of the marketed formulation showed results within acceptable limits, confirming the suitability of the method for routine quality control analysis. The proposed multivariate UV spectrophotometric method is reliable, economical, and suitable for regular analysis of Topiroxostat in pharmaceutical dosage forms.