CEACAM5/6+ Tumor Cells and IL-1β+ Macrophages Drive Resistance to Anti-PD-1-based Chemo-immunotherapy in Gastric Cancer

Processed single-cell RNA-sequencing data, immunohistochemistry whole-slide images, and prepared analysis results supporting the figures in this study. Study. The dataset covers 542,121 cells from 35 patients with advanced gastric cancer treated with anti-PD-1 plus chemotherapy, profiled before and after treatment, with matched spatial transcriptomics, immunohistochemistry, and bulk RNA-seq analyses. Contents. The record contains three logical archives. IHC_Raw_Data.zip unpacks to 02_IHC_Raw/ and includes the whole-slide scans and thumbnails. Preparation_for_Panels.zip unpacks to 02_Preparation_for_Panels/ and contains the versioned intermediate results used by the figure scripts. The 6.78-GB scRNA_H5AD_Data.zip is uploaded as six numbered parts, scRNA_H5AD_Data.zip.part00 through .part05; concatenate the parts in numerical order before unpacking it to 01_H5AD/. Reproduction. Analysis and figure-generation code is available at github.com/LiudengZhang/STAD-CEACAM-scRNA. Point STAD_RAW_INPUTS and STAD_PREPARED_INPUTS to the unpacked data and run the released workflows described in that repository. Public cohorts used in the study remain available from their original repositories and are identified in the manuscript and Supplementary Table S2.