Development And Validation of A UV Spectrophotometric Method for the Assay of Buspirone HCL Tablets

Buspirone hydrochloride is a mostly prescribed anxiolytic drug commonly available in tablet dosage forms. This study develops and validates a simple, rapid, eco-friendly UV-Visible spectrophotometric method for its quantitative analysis in solid dosage forms, meeting the criteria for practical quality control. We improved key parameters during development like wavelength selection fixed absorbance maximum at 243 nm, and solvent composition was modified to increase the sensitivity and stability utilizing quality laboratory solvent. We validated the method following ICH guidelines to confirm its reliability and repeatability. It showed strong linearity over 2.5-40 μg/mL, with a correlation coefficient r² of 0.9997. The LOD and LOQ of Buspirone HCL by the proposed method were found to be 0.094 µg/ml and 0.274µg/ml respectively, Recovery studies proved accuracy, yielding 98–102%, while precision tests confirmed excellent repeatability (RSD