Evaluation of Remineralizing Effects of Human Enamel ssing Biomimetic Waste from Nanomaterial Solutions of Fishbone(Fb) and Eggshell(Es) Incorporated with Chitosan Nanoparticles in Single Rooted Tooth - An In Vitro Study

Aim: The study aimed to evaluate the remineralizing effects of two freshly prepared organic biomimetic waste nanomaterial [fish bone (FB) and chicken eggshell (ES)] solutions on partially demineralized human enamel surface using energy dispersive X-ray spectroscopy for quantitative Ca/P assessment. Methodology: A total of 50 freshly extracted human single rooted teeth were collected to be used. The samples were divided into five equal groups (n=10) respectively. Group 1: Control group Group 2: Remineralization solution obtained from fish bone biomimetic waste Group 3: Remineralization solution obtained from chicken eggshell biomimetic waste Group 4: Remineralising solution obtained from Fishbone biomimetic waste + chitosan nanoparticle Group 5: Remineralising solution obtained from Eggshell biomimetic was +chitosan nanoparticle Results: The results shows remineralisation effects of human enamel using biomimetic wastes were significant in Group 4 remineralising solution obtained from (FISHBONE+CHITOSAN NANOPARTICLES) and least in Group 3 remineralising solution obtained from(CHICKEN EGGSHELLS) respectively.