Evaluation Of Antiangiogenic Activity of Ion Channel Modulator Capped Copper Nano Particles

Objective: The present study was undertaken to evaluate the antiangiogenic activity of copper capped 4-aminopyridine which is a potassium channel modulator in chick embryo model and zebra fish assay and also to prepare and characterize copper capped nanoparticles of 4-aminpyridine. Methods: Biosynthesis and Characterization of 4-AP copper nanoparticles was done. For the purpose Zebra fish fin regeneration and embryo assay, chick CAM asssy was performed. In this study we have selected 4-AP as the objective drug to evaluate its effect on Zebra fish fin regeneration assay and embryo assay. Results: For that purpose eggs were used and the number of branching points on 7th day was evaluated per unit area and the results were presented where the formulation 4-APCuNP have shown significant reduction in branching points from the score of 4 to 1 which is a good sign for angiogenic activity. Conclusion: Angiogenesis is one of the important factors in many physiological and pathological processes. So, control of angiogenesis using drugs is essential for homeostasis in disease conditions such as wound healing and cancer. Based on the statistical analysis, we conclude that 4-APCuNPs is good anti-angiogenic and it is more effective in inhibiting angiogenesis.