KIC Spheroid Calcium Pipeline (Script 02a/03) – Single-Batch MEANS Merging & Filtering

KIC Spheroid Calcium Pipeline — Script 02a/03 GUI-based R pipeline for merging and filtering WHOLE-IMAGE MEANS summary tables from a single CyteSeer experiment/batch generated in Script 01. What's included GUI selection of input/output paths Recursive scanning for *MEANS*.csv files Merging of per-well MEANS tables into a unified batch-level dataset Peak-range filtering or flag-only processing modes Validation of plate format (A01–H12 wells) Assignment of Group labels based on user-defined column rules Optional assignment of RowGroup labels Appending of a Batch identifier Export of a standardized merged.csv file Inputs *MEANS*.csv files generated in Script 01 Files originating from the /summary folder Required columns: Well Num.Peaks Outputs merged.csv containing: Original MEANS summary statistics Logical flags (keep_1hz, valid_well) Assigned Group (and optional RowGroup) Batch identifier Source file tracking Dataset is standardized and ready for cross-batch merging (Script 02b), statistical analysis, and downstream visualization (Script 03). Citation Developed by Michele Buono, Talitha Spanjersberg, Nikki Scheen, and Nina van der Wilt. Free for research use and adaptation with citation via Zenodo.