nf-core/rnaseq: nf-core/rnaseq v3.23.0 - Gallium Gecko

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Authors: Harshil Patel, Jonathan Manning, Phil Ewels, Maxime U Garcia, Alexander Peltzer, Rickard Hammarén, Olga Borisovna Botvinnik, Adam Talbot, Friederike Hanssen, Gregor Sturm, nf-core bot, Matthias Zepper
+18 more Denis Moreno, Ezra Greenberg, Pranathi Vemuri, Gary Burnett, Mahesh Panchal, silviamorins, Lorena Pantano, Robert Syme, EladH1, Gavin Kelly, Lorenzo Sola, James A. Fellows Yates, Matthias Hörtenhuber, Gabriel Lichtenstein, Edmund Miller, Jose Espinosa‐Carrasco, rfenouil, Luke Zappia
Cited by: 2

Abstract

What's Changed Bump version after release 3.22.2 by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1663 Update preprocessing with multiple rRNA removal tools by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1664 Add ARM containers, changelog updates, and bulk module/subworkflow updates by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1665 New module for contamination screening: Sylph by @egreenberg7 in https://github.com/nf-core/rnaseq/pull/1616 Enable SeqKit stats MultiQC module for RiboDetector rRNA removal by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1669 fix: Enhance RSEM output exports and remove unnecessary star_rsem check by @EladH1 in https://github.com/nf-core/rnaseq/pull/1667 Document star_rsem STAR aligner settings and customization by @cmatKhan in https://github.com/nf-core/rnaseq/pull/1672 Apply Nextflow 25 strict syntax fixes by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1677 fix(tests): Comprehensive PREPARE_GENOME test fixes for proper aligner coverage by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1687 feat: Migrate to topic-based version reporting by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1689 feat: Add prokaryotic RNA-seq support with -profile prokaryotic by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1688 feat: Add support for uncompressed FASTQ input files by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1686 Fix offline mode by skipping igenomes_base validation by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1696 refactor: Replace local iGenomes STAR modules with nf-core aliases by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1694 Add GPU accelerated STAR and Markdups (using Parabricks rna_fq2bam) by @gburnett-nvidia in https://github.com/nf-core/rnaseq/pull/1685 Add FastQC step after BBSplit/rRNA filtering by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1700 fix: Include single-library samples in merged fastq output by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1704 feat: Add tximport processing for RSEM outputs (#1320) by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1697 feat: Enable UMI deduplication with STAR/RSEM aligner by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1707 fix: Allow pre-built pseudo-aligner index without --fasta by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1708 docs: update metro map renders with nf-metro v0.4.7 by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1709 Fix GPU profile applying container flags globally by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1711 refactor: seq_platform/seq_center via ext.args closures by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1713 Bump version to 3.23.0 ahead of release by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1715 Fix: informative error when Salmon strandedness inference fails by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1716 Add --seq_platform parameter for global sequencing platform RG tag by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1718 Docs and changelog cleanup by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1719 Update umitools modules: remove patches, add channel prefixes by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1724 Refine .nftignore patterns and update test snapshots by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1725 docs: regenerate metro map with nf-metro v0.5.1 by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1714 docs: re-render metro map with nf-metro v0.5.4 by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1728 ci: bump Nextflow to 25.04.3 to fix conda --mkdir bug by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1730 refactor: code quality improvements from PR #1717 review by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1735 refactor: compose test_prokaryotic profile on prokaryotic by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1734 test: load prokaryotic config in nf-test instead of duplicating params by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1736 Fix iGenomes STAR conda channel for x86_64 by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1738 Dev -> master for nf-core/rnaseq 3.23.0 by @pinin4fjords in https://github.com/nf-core/rnaseq/pull/1717 New Contributors @cmatKhan made their first contribution in https://github.com/nf-core/rnaseq/pull/1672 @gburnett-nvidia made their first contribution in https://github.com/nf-core/rnaseq/pull/1685 Full Changelog: https://github.com/nf-core/rnaseq/compare/3.22.2...3.23.0

Publication details

Journal / source
Zenodo (CERN European Organization for Nuclear Research)
Publication date
27 February 2026