trippster08/Metabarcoding_on_RStudio: Metabarcoding_on_RStudio v1.0.0

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Authors: Tripp Macdonald
Cited by: 0

Abstract

I have changed the way this runs cutadqpt. Before the pipeline did not do well with mutiple gene regions that are partially overlapping and both have potential readthrough. Yes, this is a rare case, but it does happen. I've now reordered the cutadapt process, cutting 5' primers and demultiplexing first, then cutting 3' primers second, if necessary

Fields of Study & Subjects

Environmental DNA in Biodiversity Studies Genomics and Phylogenetic Studies Microbial Community Ecology and Physiology

Publication details

Journal / source
Zenodo (CERN European Organization for Nuclear Research)
Publication date
3 March 2026
Language
EN