trippster08/Metabarcoding_on_RStudio: Metabarcoding_on_RStudio v1.0.0
Abstract
I have changed the way this runs cutadqpt. Before the pipeline did not do well with mutiple gene regions that are partially overlapping and both have potential readthrough. Yes, this is a rare case, but it does happen. I've now reordered the cutadapt process, cutting 5' primers and demultiplexing first, then cutting 3' primers second, if necessary
Fields of Study & Subjects
Environmental DNA in Biodiversity Studies
Genomics and Phylogenetic Studies
Microbial Community Ecology and Physiology
Publication details
- Journal / source
- Zenodo (CERN European Organization for Nuclear Research)
- Publication date
- 3 March 2026
- Language
- EN