Fiji macro to calculate colocalisation between two point-like signals
Abstract
Fiji macro. Calculates colocalisation between two signals, based on Manders Coefficient (overlap area). Signals should be round-ish (RNA points) so we use a difference of Gaussian filter to enhance it before thresholding.CLIJ2 is used to run the difference of gaussian. Dependencies: CLIJ2 (it can be installed through the Update Site)
Fields of Study & Subjects
Cell Image Analysis Techniques
Advanced Fluorescence Microscopy Techniques
Image Processing Techniques and Applications
Publication details
- Journal / source
- Zenodo (CERN European Organization for Nuclear Research)
- Publication date
- 4 March 2026
- Language
- EN
- Document type
- Book