Gallic acid-responsive microRNAs reprogram lignification during drought acclimation process in spearmint

FOLDER: SmallRNA-20220826T155446Z-001 M2_S2_R2_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from CNT plants (replicate 2) M8_S8_R1_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from CNT-DS plants (replicate 1) M9_S9_R2_001 – smallRNA sequencing raw data from Mentha spicata leaf collected from CNT-DS plants (replicate 2) M9_S9_R1_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from CNT-DS plants (replicate 1) M11_S11_R2_001 – smallRNA sequencing raw data from Mentha spicata leaf collected from 50 µM GA-DS plants (replicate 2) FOLDER: SmallRNA-20220826T155446Z-003 M1_S1_R1_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from CNT plants (replicate 1) M1_S1_R2_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from CNT plants (replicate 2) M3_S3_R2_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from CNT plants (replicate 2) M5_S5_R2_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from 50 µM GA plants (replicate 2) M6_S6_R1_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from 50 µM GA plants (replicate 1) M6_S6_R2_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from 50 µM GA plants (replicate 2) FOLDER: SmallRNA-20220826T155446Z-002 M2_S2_R1_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from CNT plants (replicate 1) M3_S3_R1_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from CNT plants (replicate 1) M4_S4_R1_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from 50 µM GA plants (replicate 1) M4_S4_R2_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from 50 µM GA plants (replicate 2) M8_S8_R2_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from CNT-DS plants (replicate 2) M10_S10_R1_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from 50 µM GA-DS plants (replicate 1) M10_S10_R2_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from 50 µM GA-DS plants (replicate 2) M11_S11_R1_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from 50 µM GA-DS plants (replicate 1) M12_S12_R1_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from 50 µM GA-DS plants (replicate 1) M12_S12_R2_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from 50 µM GA-DS plants (replicate 2) FOLDER: SmallRNA-20220826T155446Z-004 M5_S5_R1_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from 50 µM GA plants (replicate 1) M7_S7_R1_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from CNT-DS plants (replicate 1) M7_S7_R2_001 - smallRNA sequencing raw data from Mentha spicata leaf collected from CNT-DS plants (replicate 2) GENERAL EXPERIMENTAL CONDITIONS One-month-old spearmint plants (Mentha spicata subsp. spicata L.) were divided into two groups: control (CNT) and test. Pure gallic acid (GA) was mixed with the mineral soil of the test plants (8.50 mg per Kg of soil; henceforth, 50 µM GA). A set of control and test plants (henceforth, CNT and 50 µM GA) were irrigated daily to maintain a water level equal to 85% of the field capacity (FC), while another set was subjected to a regime of drought stress (henceforth, DS; CNT-DS and 50 µM GA-DS) consisting in a suboptimal watering (42.5% FC). The experiment was carried out for 21 days (temperature: 22 °C; photoperiod 14 h light/10 h dark; homogeneous light intensity: 120 μmol m-2 s-1); after that microRNAs were extracted from leaf tissues.