HOT-LAVA - KOK1806 - HPLC

These Simons Collaboration on Ocean Processes and Ecology (SCOPE) data products were produced by the University of Hawaiʻi at Mānoa with support from the Simons Foundation. This dataset was collected on the R/V KOK for the HOT-LAVA cruise to Hawaiʻi Island in the north Pacific Ocean. This cruise sampled a chlorophyll bloom induced by volcanic lava reaching the ocean during the 2018 Lower Puna eruption. Samples were processed to detect and quantify phytoplankton pigments using a modified version of the high-performance liquid chromatography (HPLC) method of Wright et al. (1991) as described by Bidigare et al. (2005). HPLC samples were collected from CTD niskin bottles and filtered onto Whatman GF/F filters and stored at -80°C until analysis. Filters were extracted in 100% acetone for 24 h at 4°C. Extracts were processed in reverse-phase on a 250 mm C18 column using a Waters separations module and photodiode detector array. Empower chromatography software was used for integration and pigment quantification. The instrument was calibrated using chlorophyll a from Anacystis nidulans (Sigma USA) and mixed pigment standards were obtained from DHI in Denmark. Timestamps are in UTC time.