ABSORBANCE CORRECTION UV SPECTROSCOPIC METHOD FOR SIMULTANEOUS ESTIMATION OF CLOPIDOGREL BISULPHATE AND RIVAROXABAN

A reliable and cost-effective testing method for Clopidogrel bisulphate and Rivaroxaban drugs. The method uses the absorbance correction principle which depends on different absorbance patterns of the two drugs at specific wavelengths. This method enables precise drug measurement because it does not need any separation procedure. Clopidogrel bisulphate reached its highest absorbance level at 204 nm while Rivaroxaban displayed its unique absorbance peak at 250 nm. Researchers calculated drug concentrations by observing absorbance at these specific wavelengths and using appropriate absorbance correction equations. When methanol was used as the solvent for testing, both analytes demonstrated conformity with Beer-Lambert's law between concentration limits of 2-12 μg/mL. The ICH Q2 (R2) requirements were followed in the validation of the analytical method. Parameters linearity, precision, limit of detection, limit of quantification, and accuracy were assessed during the validation procedure. According to the study, there was a clear correlation between the concentration levels of both medications and absorbance measures, which yielded findings that were very similar to a value of one. Because the percentage recoveries fell within acceptable bounds, recovery experiments validated the method's accuracy. According to the precision investigations, %RSD values remained below 2% which showed that the test results achieved both repeatability and intermediate precision. The developed absorbance correction method is therefore suitable for routine quality control analysis.