Automated Peak Detection and Proximity Analysis of Dual-Channel Intensity Profiles

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Authors: Devin Frisby
Cited by: 0

Abstract

This R script performs automated peak detection and cross-channel proximity analysis for paired fluorescence intensity profiles. The script identifies peaks in a primary signal (green channel), filters peaks based on an intensity threshold, and determines whether those peaks spatially coincide with signal above threshold in a secondary channel (magenta) within a defined angular window. The script is designed for circular intensity profiles (e.g., line scans around vesicles, endosomes, or other ring-like structures) and includes wrap-around indexing to correctly handle peaks near the start/end of the angular range.

Fields of Study & Subjects

Cell Image Analysis Techniques Advanced Fluorescence Microscopy Techniques Cellular transport and secretion

Publication details

Journal / source
Zenodo (CERN European Organization for Nuclear Research)
Publication date
6 March 2026
Language
EN