Automated Peak Detection and Proximity Analysis of Dual-Channel Intensity Profiles
Abstract
This R script performs automated peak detection and cross-channel proximity analysis for paired fluorescence intensity profiles. The script identifies peaks in a primary signal (green channel), filters peaks based on an intensity threshold, and determines whether those peaks spatially coincide with signal above threshold in a secondary channel (magenta) within a defined angular window. The script is designed for circular intensity profiles (e.g., line scans around vesicles, endosomes, or other ring-like structures) and includes wrap-around indexing to correctly handle peaks near the start/end of the angular range.
Fields of Study & Subjects
Cell Image Analysis Techniques
Advanced Fluorescence Microscopy Techniques
Cellular transport and secretion
Publication details
- Journal / source
- Zenodo (CERN European Organization for Nuclear Research)
- Publication date
- 6 March 2026
- Language
- EN