Spatial Cytotoxic–Stromal Interaction Field: An Analytical Framework for Tumor Microenvironment Dynamics

Experimental research note This manuscript presents an experimental theoretical framework currently under active development.The concepts, terminology, and variable definitions are still being consolidated, and version consistency across related manuscripts has not yet been fully standardized. The present document integrates elements from a prior theoretical note and several tumor-specific analyses.It is released here for transparency and early documentation of the conceptual development process. This version should therefore be regarded as a working research manuscript, not a finalized publication.Subsequent versions may revise terminology, parameters, and structural definitions as the framework evolves. Spatial Cytotoxic–Stromal Interaction Framework This repository introduces a theoretical and analytical framework for quantitative characterization of immune–stromal organization within tumor microenvironments using spatially resolved data. Earlier studies explored immune resistance through temporal and spatiotemporal coordination models. The research program evolved through several conceptual stages: temporal coordination hypotheses (∆t; v2.x), spatiotemporal accessibility models (v3.0), dynamic coordination formulations (v3.3), and finally the spatial interaction field framework developed in the present work. The current manuscript focuses on spatial interaction structure derived from transcriptomic signals. Earlier exploratory analyses, including the v4.x studies, represent intermediate stages in the development of the CIF framework. These analyses were conducted prior to the establishment of standardized parameter conventions and preprocessing definitions. Parameter definitions and preprocessing procedures were consolidated only in the present manuscript and accompanying appendices. Consequently, baseline parameter settings and analytical conventions used in earlier exploratory studies may differ from those specified in the current implementation. The repository includes the main manuscript describing the theoretical formulation together with supplementary appendices and analytical scripts used for computational evaluation. Appendix A provides terminology clarification to ensure consistent notation across the manuscript and analytical code.Appendix B documents preprocessing procedures, spatial smoothing definitions, and implementation details supporting computational reproducibility. All materials are intended as a conceptual and analytical foundation for further investigation using spatially resolved tumor transcriptomic datasets.