ACTIVATION OF GRANULOCYTE MITOCHONDRIA DURING INCUBATION WITH QUERCETIN: ASSESSMENT OF FUNCTIONAL ACTIVITY IN VITRO

Quercetin is a flavonoid with antioxidant properties and the ability to modulate cellular energy metabolism by activating mitochondrial biogenesis. However, the effect of this compound on the functional activity of granulocytes remains insufficiently studied. The aim of this study was to evaluate the effect of quercetin on the functional activity of neutrophils (protein metabolism, mitochondrial activity, oxidative stress, oxygen metabolism, and phagocytosis) in vitro and to substantiate its mechanism of action as a modulator of mitochondrial function. The functional activity of neutrophils in venous blood samples from 20 healthy donors was assessed by luminescence microscopy before and after incubation with quercetin (10 μM, 1 hour, 38°C). The NADH level was determined by the intensity of autofluorescence in the UV range. Statistical processing was performed using the paired Student's t-test (p