Dematipyriforma aquilariae L. Y. Sun, Hai Y. Li, Xiang Sun & L. D. Guo

Dematipyriforma aquilariae L. Y. Sun, Hai Y. Li, Xiang Sun & L. D. Guo Fig. 4 Description. Saprobic on dead, decaying stems of Typha sp. Sexual morph: Undetermined. Asexual morph: Hyphomycetous. Colonies black, effuse, sporodochial. Mycelium 1.2–2.5 μm (x ̄ = 2 μm, n = 30), immersed, smooth, subhyaline to pale brown, branched, septate, thin-walled. Chlamydospores 4–5 × 6.5–8.5 μm (x ̄ = 4.5 × 7 μm, n = 30), intercalary or terminal, solitary, catenated, straight, or curved, brown to dark brown with a pale intermediate cell, smooth walls thickened with axial performative canals 1–8 transverse and occasionally 1 or more oblique or longitudinal septa, constricted at the septa. Conidiophores 2–6 μm wide (x ̄ = 4.5 μm, n = 10), micronematous, mononematous, subhyaline to pale brown, smooth, straight to flexuous, septate. Conidiogenous cells 4.5–6 × 5.5–8 μm (x ̄ = 5.5 × 7.8 μm, n = 30), holoblastic, integrated, intercalary, determinate, cylindrical, pale brown to brown with thin walls, conidial secession rhexolytic. Conidia 24–30 × 15–20 μm (x ̄ = 26.5 × 17.5 μm, n = 30), pyriform or elongated pyriform, solitary, intercalary, smooth, thin-walled, muriform, rounded at the apex, pale grey olivaceous to pale brown, acropetal pigmentation during conidium formation. Culture characteristics. Conidia germinating on malt extract agar (MEA) within 24 h. Germ tube produced from the base of conidia. Colonies growing on MEA, reaching 40 mm in 4 weeks at 25 ° C. Mycelia superficial, effuse to flat or undulate, from above olivaceous to greyish canter to the complete edge, from reverse greenish-black at the canter, light brown to black to hyaline edge. Material examined. Thailand • Prachuap Khiri Khan Province, Pran Buri District, Pran Buri Wetland, on decaying stems of Typha sp. (Typhaceae), 25 August 2023, Tharindu Bhagya, TB 113 (MFLU 25-0195), living culture (MFLUCC 25-0242). GenBank numbers. ITS = PV 759789, LSU = PV 759788, SSU = PV 759791, rpb 2 = PV 779202. Notes. Dematipyriforma aquilariae (MFLU 25-0195) has a phylogenetic affinity with D. aquilariae (CGMCC 3.17268) and clusters with 100 % ML and 1.00 BYPP support (Fig. 1). The new isolate agrees with the general description of Dematipyriforma by possessing monoblastic integrated conidiogenous cells and pyriform conidia with transverse, oblique or longitudinal septation (Sun et al. 2017; Bao et al. 2022). Dematipyriforma aquilariae (MFLU 25-0195) and D. aquilariae (CGMCC 3.17268) have rhexolytic conidial secession, smooth and thin-walled, pyriform or elongated pyriform, dark brown to black conidia with acropetal pigmentation (Fig. 4; Sun et al. 2017; Bao et al. 2022). Considering both phylogenetic and morphological evidence, we identify MFLU 25-0195 as a new isolate of D. aquilariae, and report this as a new host record for Typha sp., from freshwater coastal wetlands of Thailand.