Confocal scanning laser microscopy images of Fluo-4 AM and CellBrite Fix 555 stained B. subtilis incubated on nanostructured calcium deficient hydroxyapatite substrates

Figure 4. Quantification of intracellular calcium in the bacterial population adhered to the substrates by Fluo4-AM Bacteria Bacillus sibtilis imaged directly on CDHA surfaces by confocal miscroscopy using Fluo4AM staining. 3h incubation in PBS. Raw confocal images with metadata and the corresponding images exported in .png format. .czi images can be opened with Zeiss Zen Blue/Black software and FIJI.