Technical Bulletin for GROQ-seq TEV Protease Function Assay

Zenodo (CERN European Organization for Nuclear Research) 2026 Open access

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Authors: Dana L. Cortade, James R. McLellan, Catherine Baranowski, Amanda Apel, Peter J. Kelly, Aviv Spinner, Zach Sisson, Andi Dhroso, Corey Hudson, Anjali Chadha, Erika Alden DeBenedictis, Svetlana Ikonomova
+1 more D. L. Ross
DOI: 10.5281/zenodo.19551806
Cited by: 1

Abstract

This technical bulletin presents a pooled assay for measuring tobacco etch virus (TEV) protease function using the GROQ-seq platform and its first generated dataset. The assay links enzyme function to cellular fitness via antibiotic resistance, enabling high-throughput functional profiling by sequencing. The resulting GROQ-seq TEV Protease assay produces calibrated function measurements spanning an approximate 100-fold dynamic range. Its first generated dataset includes over 18,000 TEV protease variants across 24 different experimental conditions. This work and resulting dataset support the development of predictive models for assessing mutational effects.

Fields of Study & Subjects

Viral Infectious Diseases and Gene Expression in Insects Molecular Biology Techniques and Applications Evolution and Genetic Dynamics

Publication details

Journal / source
Zenodo (CERN European Organization for Nuclear Research)
Publication date
13 April 2026