Spectrum-FISH: Sequencing-free spatial profiling of post-transcriptional regulation in fresh tissues

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Authors: Xianglin Ji, Youyang Wan, Guo F
Cited by: 1

Abstract

This dataset is associated with the research paper “Sequencing free spatial profiling of post transcriptional regulation in fresh tissues.” It contains multiple files corresponding to different biological samples and demonstration datasets. The .mat files are MATLAB data files provided for demonstration using rodent tissues. The mRNA-related files (mRNA_data_E9.mat, mRNA_data_E10.5.mat, and mRNA_data_E12.mat) correspond to spatial profiling of embryonic neural tube tissues at different developmental stages. The m6a_data.mat and miRNA_data.mat files are associated with studies of olfactory bulb tissues, focusing on m6A-related and miRNA-related post-transcriptional regulation, respectively. The CRC20.h5ad file is related to a study using human biopsy tissues. The .zip file contains raw fluorescence images for demonstration of decoding the 4-channel Spectrum-CODE system. The accompanying text files, such as Readme.txt and h5adloading.txt, provide instructions for loading the data.

Publication details

Journal / source
Zenodo (CERN European Organization for Nuclear Research)
Publication date
22 April 2026
Document type
Dataset