Yildiz-Lab/YFIESTA: v1.1.1

Yildiz Lab FIESTA Adaptations This repository contains adaptations developed by the Yildiz Lab to the base tracking software FIESTA, originally introduced by Ruhnow et al. (2014)00467-X?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS000634951100467X%3Fshowall%3Dtrue). Overview These modifications extend FIESTA's capabilities for high-precision single-molecule tracking and downstream analysis, particularly for MINFLUX-based datasets and step detection workflows. Key Features MINFLUX trace support Load and visualize MINFLUX trajectories directly within the GUI framework. Interactive step-finding GUI Located in the FIONAviewer subfolder, enabling manual and semi-automated step detection. Enhanced step analysis tools Support for both 1-color and 2-color trajectory analysis, including step size distributions. Implemented in the stepping histogram distribution subfolder and integrated with FIONAviewer. Software Requirements MATLAB: Developed and tested in MATLAB R2025a Associated Work This software was used in: Slivka et al., 2026 Zhao et al., 2023 License MIT License Copyright (c) 2026 Yildiz Lab Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.