Head-to-Head CRISPRko CRISPRi Perturb-seq: Aligned, filtered data with guide assignments

Laura Drepanos; Michael Gatzen; Berta Escude Velasco; Abigail Chase; John G. Doench

doi:10.5281/zenodo.20722064

Head-to-Head CRISPRko CRISPRi Perturb-seq: Aligned, filtered data with guide assignments

Zenodo (CERN European Organization for Nuclear Research) 2026 Dataset Open access

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Authors: Laura Drepanos, Michael Gatzen, Berta Escude Velasco, Abigail Chase, John G. Doench

DOI: 10.5281/zenodo.20722064

Cited by: 1

Abstract

Low MOI perturb-seq data (as Seurat objects) from parallel Cas9 CRISPRko and CRISPRi benchmark libraries. Fastqs were aligned with Illumina DRAGEN software, which filters droplets to "passing cells". Cells are further filtered to those with

Publication details

Journal / source: Zenodo (CERN European Organization for Nuclear Research)
Publication date: 16 June 2026
Document type: Dataset