Supplementary material for "eIFiso4G editing confers high and broad-spectrum resistance against rice yellow mottle virus"

Sup. Data 1 - Sequences of the eIFiso4G1 and eIFiso4G2 protein in the Kitaake variety and in the different mutant lines.Sup. Table 1 - Number and genotypes of T0 and T2 plants obtained with the different constructs. Sup. Table 2 - Segregation of KO and WT alleles in F2 populations derived from crosses between OseIFiso4G1 KO and OseIFiso4G2 KO lines.Sup Table 3 - Grain filling rate in F2 plants and germination rate in F3 progenies derived from crosses between OseIFiso4G1 KO and OseIFiso4G2 KO lines.Sup. Table 4 - Resistance-breaking rates on OseIFiso4G1 KO lines.Sup. Table 5 - Residues involved in key salt-bridges within the VPg central helix–MIF4G hairpin complex.Sup. Table 6 - Summary of phenotypic traits in the edited lines.Sup. Fig. 1 - Expression level of OseIFiso4G1 in lines with deletions in the central domain of the gene.Sup. Fig. 2 - Resistance of edited lines against BF1 isolate of RYMV.Sup. Fig. 3 - Resistance of edited lines against isolates CIa, Ng106, Mg1 and Tz11.Sup. Fig. 4 - Morphology of edited lines in controlled conditions.Sup. Fig. 5 - Comparison between the predicted 3D model of the rice MIF4G domain and the crystallographic structure of human MIF4GII.Sup. Fig. 6 - MIF4G 3D models of WT Kitaake and edited variants.Sup. Fig. 7 - Predicted 3D structure of MIF4G/VPg complex for WT Kitaake and edited variants.Sup. Fig. 8 - Conformational variability in the predicted 3D structures of the MIF4G domain for the rymv1-3 allele.