AnniWarn/Nox2: Nox2

Initial release of the neutrophil Nox2 image-analysis pipeline. This software supports the quantitative analysis of microscopy data. The release includes the python script to calculate colocalisation of Nox2 subunits in isolated mouse and human neutrophils, and CellProfiler pipeline to quantify protein expression in the Ly6G-Cre Nox2 fl/fl mouse model. The workflow was developed for the analysis described in the manuscript "Neutrophil NADPH oxidase breaks the inflammatory IL-1β/IL-17A circuit to enhance pathogen clearance during respiratory virus infections." Version: 1.0.0 Requirements: Fiji, CellProfiler, JupyterLab Python 3.9 Associated publication: Sofoluwe A, Petropoulos A, Ali-Khan ZF, Goomanee S, Cleary SJ, Warnatsch A, Neutrophil NADPH oxidase breaks the inflammatory IL-1β/IL-17A circuit to enhance pathogen clearance during respiratory virus infections. Nature Communications