Serial Coronal Sections Of An Adult Mouse Brain - Sample Dataset

Experimental Data:Serial coronal sections of the brain of an adult mouse. Sectioning and Imaging:20 µm brain sections sampled every 80 µm, imaged on an Olympus VS120 slide scanner with a 10x objective. Channels:The dataset includes label and overview images, as well as three fluorescent channels: DAPI channel: Cell nuclei FITC channel: Autofluorescence Cy3 channel: Fluorescent sparse mCherry-labeled cells Use Case:This dataset can serve as a test dataset for the Aligning Big Brain and Atlases tool. Ethics:Animal handling was conducted according to protocols approved by the Swiss animal license VD2808.2. QuPath Projects:Two zipped QuPath projects utilizing the OMERO extension are provided: One contains the full dataset of 97 sections. The other contains a subset of 25 sections.These data are hosted on the German BioImaging public OMERO instance (link). Additional Data:This dataset also includes tiles of a detailed 3D image of the dentate gyrus from a mouse brain section (Swiss animal license VD3662). This section was imaged using a Leica SP8 with a 60x objective (NA 1.40). Note that this detailed region originates from a different dataset than the one imaged with the slide scanner. Channels: DAPI eGFP