Effects of lactation period on some blood parameters and ANAE/AcP-ase enzyme activities in domestic Donkeys

It was aimed to research the influences of the lactation period on some hematological (red blood cells, white blood cells, and its subtypes, hematocrit, hemoglobin, mean erythrocyte hemoglobin, mean corpuscular hemoglobin concentration, mean erythrocyte volume, and blood clot count) and biochemical parameters (alanine amino transferase, aspartate amino transferase, gamma glutamyl transferase, blood urea nitrogen, and creatinine) as well as α - naphthyl acetate esterase and activity of the enzymes acid phosphatase positive lymphocytes percentages in domestic donkeys. For this purpose, 20 female donkeys were selected and divided into two equal groups as control (non-lactating, n = 10) and lactating (n= 10). They were in the middle of lactation (three to six months old), ranging in age from five to fourteen. Following the routine clinical examination, blood samples were taken from the animals in order to measure the activities of the α - naphthyl acetate esterase / activity of the enzymes acid phosphatase enzyme as well as several blood parameters. As a result, red blood cells, hematocrit, and hemoglobin values were higher in lactating donkeys than non-lactating donkeys (P < 0.05). Serum aspartate amino transferase and gamma glutamyl transferase values were found to be statistically higher in lactating animals (P < 0.05). Although enzyme activities were high in lactating donkeys, α - naphthyl acetate esterase and activity of the enzymes acid phosphatase positive lymphocyte percentages were found to be similar between the groups (P > 0.05). In conclusion, the middle-lactation phase in female donkeys was found to have an impact on several blood parameters, but not on the activity of enzymes.