Study on the Effect of AtTTG1 on Salt Tolerance of Arabidopsis Thaliana

Soil salinization, caused by natural processes or human activities, is characterized by excessive soluble salts in the soil. It severely impairs normal plant growth and development. The TRANSPARENT TESTA GLABRA1 (TTG1) gene encodes a WD40 repeat protein that participates in multiple key biological processes, including trichome development, cuticular wax biosynthesis, photosynthetic efficiency regulation, and salt stress response. These functions enable TTG1 to support the growth adaptation of Arabidopsis thaliana under different environmental conditions. In this study, CRISPR-Cas9-mediated TTG1 knockout lines of A. thaliana, generated in a previous study, were used as experimental materials. In this study, CRISPR-Cas9-generated TTG1 knockout lines (from a prior experiment) were used as experimental materials. Genomic DNA was extracted from candidate lines, followed by nested PCR validation and Sanger sequencing to eliminate false-positive individuals. Phenotypic characterization of confirmed positive mutants was conducted to dissect the role and regulatory mechanism of TTG1 in plant salt stress tolerance.