USE OF EBAP MARKERS FOR IDENTIFICATION AND DIFFERENTIATION OF OLD MAIZE GENOTYPES

Knowledge of the genetic diversity of corn is important in the corn breeding process. This study was conducted to determine genetic diversity among 40 old maize genotypes using 36 EBAP primers and to verify the usability of these primers for the study of maize polymorphism. All 36 EBAP primers have amplified products, with the highest number of fragments provided by primer EBAP18 (14) and the lowest number of fragments provided by primers EBAP5, EBAP11, EBAP12 and EBAP 31. The total average number of fragments per primer was 6.75. The number of polymorphic fragments using 36 EBAP primers ranged from 13 (EBAP18) to 1 (EBAP3, EBAP5, EBAP12, EBAP20, EBAP23, EBAP28, EBAP31 and EBAP33) with an appropriate number of 4.36 polymorphic fragments per primer. The percentage of polymorphism of the analyzed fragments ranged from 100% (EBAP4, EBAP 27 and EBAP36) to 16.67% (EBAP3 and EBAP28) with an average value of 57.94%. For each primer was calculated the values of index: TNB, NPB, PPB, Na, Ne, H, I, PI, Rp, PIC, MI. By using 36 EBAP primers, we managed to divide the 40 old analyzed maize genotypes into 2 main clusters (cluster 1 and cluster 2), while 2 genotypes from Poland (Przebedowska Biala and Przebedowska Burskynowa) were separated separately in the obtained dendrogram. Based on the obtained results, we confirmed the usability of EBAP primers for the study of polymorphism of old maize genotypes.