Characterization of Green Synthesized Silver Nanoparticles from Clerodendrum thomsoniae Balf.f., and Their Antioxidant and Anti inflammatory Potential

The most promising nanomaterials for biological applications in recent years are silver nanoparticles (AgNPs), especially as innovative antibacterial, antioxidant, anticancer, larvicidal, catalytic, and wound healing activities 5 .Secondary metabolites from medicinal plants, such as sugars, polysaccharides, alkaloids, and flavonoids, have been shown to be quite useful when synthesizing nanoparticles.The genus Clerodendrum has been implicated in various traditional medicine systems for the treatment of various life-threatening diseases.The medicinal plant Clerodendrum thomsoniae Balf.f., often known as the bleeding-heart vine or bag flower, is an ornamental vine used in Indian and Japanese traditional systems to treat a variety of severe ailments, such as cancer, typhoid, syphilis, jaundice, and hypertension 6 .It is a member of the genus Clerodendrum and the family Lamiaceae 6 .Clerodendrum thomsoniae is found in large numbers in America, Africa, Australia, and Asia 7 .Traditional healers in Cameroon employ C. thomsoniae as a folk medicine to cure obesity and diabetes.Fatty acids (linoleic acid, palmitic, and heptadecanoic acid) and phytosterols (stigmasterol and α-tocopherol) are among the medicinally important phytocompounds in C. thomsoniae 8 .A sustainable method for the biosynthesis of silver nanoparticles (AgNPs) using C. thomsoniae leaf extract is being developed in light of the plant's immense medicinal value.The produced AgNPs were characterized using UV, ABSTRACT Introduction: Medicinal plant extracts and other natural products have continued to find useful applications in nanomedicines due to their interesting biological properties.Clerodendrum thomsoniae (CT) is a plant used in traditional medicine to treat stress-and inflammation-related diseases, including jaundice, diabetes, and cancer.Objectives: This study, therefore, evaluated CT extract-based silver nanoparticles (Ag NPs) for their antioxidant and anti-inflammatory potential.Materials and Methods: The nanoparticles were prepared using green synthesis methods.They were characterized using UV-Vis spectroscopy, scanning electron microscopy (SEM), field emission scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD).Antioxidant study was based on NO, H2O2, superoxide, and hydroxyl radical scavenging spectrophotometric methods.The in vitro anti-inflammatory test was based on a protein (egg albumin) denaturation assay.Results: Results showed CT-Ag NPs ranged from spherical to cubic shapes.The UV absorption peak at 427 nm suggests CT-Ag NP formation.The presence of elemental Ag (96.04 %) by EDX analysis suggests the conversion of metallic silver into elemental silver.The crystallinity of the nanoparticles was shown on the X-ray diffractogram as a sharp peak at 38.12° [reflection index (111)] with an average particle size of 47 nm.CT-Ag NPs showed dose-dependent hydroxyl and nitric oxide radical scavenging activities with 67.63 ± 0.78 % and 58.48 ± 1.20 %, respectively, at 200 μg/mL.It showed a notable anti-inflammatory effect by inhibiting protein denaturation with an IC50 of 53.58 ± 17.78 μg/mL.Conclusions: It can be deduced from this study that CT-Ag NPs show promise as antioxidant and anti-inflammatory agents.