Hepatoprotective Potential of Coffea arabica Extract Against Carbon Tetrachloride Induced Liver Damage: Ultrasonographic and Cytokine Evaluation

Carbon tetrachloride (CCl₄) is a hepatotoxic agent widely used to model liver injury in experimental animals, and coffee (Coffea arabica) extract is known to contain various bioactive compounds with antioxidant and anti-inflammatory potential.This study aimed to evaluate the hepatoprotective activity of Coffea arabica extract in male Wistar rats induced with CCl₄ through phytochemical analysis, liver biochemical parameters (albumin, bilirubin, SGOT, SGPT), inflammatory cytokines (TNF-α, IL-6, CRP), and liver morphology observations using ultrasonography and histopathology.The extract contained alkaloids, flavonoids, triterpenoids, saponins, and glycosides, with total phenolic and flavonoid contents of 51.32 mg GAE/g and 1.25 mg QE/g extract, respectively, and moderate antioxidant activity (IC₅₀ = 124.36ppm).Administration of the extract at 300 mg/kg BW significantly improved liver structure, reduced TNF-α (120.62 ± 21.78 pg/mL), IL-6 (68 ± 14.44 pg/mL), CRP (199.0 ± 41.5 pg/mL), SGOT (147.0 ± 34.28 g/dL), and SGPT (69.6 ± 9.94 g/dL) levels, while increasing albumin (2.94 ± 0.34 g/dL) and reducing bilirubin (0.09 ± 0.04 mg/dL), approaching normal conditions.The 300 mg/kg BW dose showed the most optimal protective effect compared to other treatment groups, as evidenced by improvements in biochemical parameters, liver morphology, and hepatic histology, indicating that Coffea arabica extract has strong potential as an effective hepatoprotective agent against CCl₄-induced liver damage.