Association Of IL-4 Gene Polymorphism with Serum Ige And Eosinophil Levels in Asthma Patients

Asthma represents a chronic airway inflammatory disease with a Th2-cell polarization as the major design of immune response, and interleukin-4 (IL-4) is pivotal in IgE production and eosinophilia. Polymorphisms in the IL-4 gene can alter an individual's susceptibility to asthma and its associated immunological markers. The purpose of this study was to determine if there was a relationship between the genotypic distribution in 2 IL-4 gene polymorphic sites and the asthma IgE level and the peripheral eosinophil count. A cross-sectional case-control study was carried out at Al-Sadr Medical City, Najaf, Iraq from March to December 2025, that included 98 asthmatic patients who were diagnosed by the Global Initiative for Asthma (GINA) guidelines and 52 healthy controls age- and sex-matched. Demographic and clinical information was obtained with the help of structured questionnaires. Serum total IgE were quantified by ELISA, eosinophils were determined with an automated hematology analyzer and the IL-4 gene polymorphism was investigated using PCR-RFLP. Serum IgE levels (310.6 ± 118.4 IU/mL vs. 142.3 ± 64.7 IU/mL, P < 0.002) and eosinophil counts (468.2 ±152.6 vs.286·9 _. 104.s cellsip1L, :P