Design and fabrication of Petri dish optimized for high-frequency acoustic microscopy using 3D printing

ABSTRACT High-frequency imaging in acoustic microscopy requires the use of petri dishes with thin and acoustically transparent bottoms to minimize signal attenuation and distortion. Commercially available options suitable for this purpose are often expensive or limited in compatibility with custom setups. Here, we present a cost-effective and easily customizable alternative developed in our laboratory, consisting of a 3D-printed frame combined with commercially available stretch film. The stretch film serves as an ultrathin (8 µm thickness) base that supports efficient high-frequency acoustic transmission, enabling clear and reliable imaging. Beyond acoustic microscopy, the adaptable design is also compatible with other modalities such as optical tweezers and fluorescence microscopy. This versatility significantly enhances the value of the proposed design, offering a practical solution for diverse experimental needs.