Diethyl-2-(4-nitrobenzyliden)malonate synthesis and it’s application for 2-methylmercaptoacetate detection by HPLC method
Abstract
The basic requirements for an effective reagent in the pre-column derivatisation of organic substances with no chromophores are outlined. The study examined nitrofluorostyrenes, dichlorostyrenes, and diethyl-2-(4-nitrobenzylidene)malonate as potential new reagents. The latter was selected as the optimal reagent. The derivatisation of 2-methylmercaptoacetate as a test analyte was performed under mild conditions (20 minutes at room temperature). Complete reaction with formation of a single product was observed. The detection limit of 2-methylmercaptoacetate decreased 67-fold (to 15 ng/mL) compared to the initial limit.
Fields of Study & Subjects
Analytical Chemistry and Chromatography
Analytical Methods in Pharmaceuticals
Analytical chemistry methods development
Publication details
- Journal / source
- Lomonosov chemistry journal
- Volume
- 67
- Issue
- 2026-2
- Pages
- 83–83
- Publication date
- 12 February 2026
- Language
- EN