Extraction and Characterization of Hedyotis purpurascens Hook.f. Ethanolic Leaf Extract by Using HPLC and FTIR Techniques

Medicinal plants remain a vital reservoir of structurally diverse bioactive compounds with significant therapeutic potential. In this context, the present study aimed to explore and analyze the phytochemical profile of Hedyotis purpurascens Hook.f. through advanced analytical techniques. The ethanolic leaf extract was subjected to High-Performance Liquid Chromatography (HPLC) and Fourier Transform Infrared Spectroscopy (FTIR) to establish a reliable chemical fingerprint. HPLC analysis, performed using Ampelopsin, quercetin, and myricetin as reference standards, revealed twelve distinct chromatographic peaks. A prominent peak at a retention time of 4.919 min closely matched that of Ampelopsin (4.992 min), strongly indicating its presence in the extract. This comparative chromatographic evaluation enhances the precision of compound identification and ensures reproducibility, which is crucial for quality control. Complementary FTIR analysis further confirmed the existence of key functional groups, including phenolic, aromatic, carbonyl, amine, and alkane moieties, reflecting the extract’s rich phytochemical complexity. The integration of HPLC and FTIR methodologies provides a robust, reproducible, and scientifically validated chemical signature for H. purpurascens. These findings not only substantiate its phytochemical diversity but also lay a strong analytical foundation for future pharmacological validation, standardization, and the development of evidence-based herbal formulations.