Synthesis and Characterization of Titanium Dioxide Nanoparticles from Ethanol Extracts of Portunus pelagicus (Linnaeus, 1758) and Their Evaluation of Brine Shrimp Lethality Assay

Aim: The present study focusses on the synthesis of titanium dioxide nanoparticles from the shell and the whole-body tissue ethanolic extracts of the blue crab Portunus pelagicus (Linnaeus, 1758). Materials and Methods: The nature and characteristics of the synthesized nanoparticles were analysed using various techniques including XRD, FTIR, SEM, EDS and UV-visible spectroscopy. The brine shrimp lethality assay of the synthesized nanoparticles was also evaluated. Results: The results revealed that the nanoparticles were spherical in shape, exhibited a tetragonal structure with an anatase phase, and were confirmed as titanium dioxide nanoparticles by EDS analysis. UV-visible spectral studies indicated a band gap energy of 3.14 eV. The brine shrimp lethality assay of the nanoparticles synthesized from ethanolic extract of shell demonstrated insignificant mortality at low doses with only a slight increase at 1500 µg/ml, while the nanoparticles synthesized from ethanolic extract of tissue exhibited dose dependent cytotoxic activity. Conclusion: Thus, the synthesized titanium dioxide nanoparticles exhibit promising potential for diverse applications.