SYNTHESIS OF PYRAZOLINES FROM HYDRAZINE HYDRATE, AND EVALUATION BY ANTIINFLAMMATORY ACTIVITY

Pyrazolines are an important class of nitrogen-containing heterocycles with diverse pharmacological properties, particularly anti-inflammatory activity.In this study, a series of 1,3,5-trisubstituted pyrazoline derivatives were synthesized via cyclization of chalcones with hydrazine hydrate under reflux conditions.The chalcones were initially prepared through Claisen-Schmidt condensation of substituted acetophenones and aromatic aldehydes.The structure of the synthesized pyrazolines was confirmed by spectral characterization using FTIR, ^1H NMR, ^13C NMR, and mass spectrometry, which validated the formation of the pyrazoline ring and key functional groups.The anti-inflammatory potential of the synthesized compounds was evaluated using the in vitro protein denaturation assay and in vivo carrageenan-induced paw edema model in rats.Several derivatives exhibited significant anti-inflammatory activity, comparable to standard drugs such as diclofenac, suggesting a strong correlation between structural features and biological efficacy.The results indicate that electron-donating and electron-withdrawing substituents on the aromatic ring influenced the degree of activity.This study highlights the therapeutic promise of pyrazoline scaffolds and provides a foundation for the development of novel anti-inflammatory agents.