DEVELOPMENT AND VALIDATION OF ANALYTICAL RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF ANTI-HYPERTENSIVE AGENTS EFONIDIPINE AND TELMISARTAN IN BULK AND MARKETED PHARMACEUTICAL DOSAGE FORM

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of Efonidipine and Telmisartan in pure form and marketed combined pharmaceutical dosage forms.A column having Symmetry ODS (C18) (250mm x 4.6mm, 5µm) in isocratic mode with mobile phase containing Acetonitrile: Phosphate Buffer (pH-3.2) (35:75% v/v) was used.The flow rate was 1.0 ml/min and effluent was monitored at 252 nm.The retention time (min) and linearity range (ppm) for Efonidipine and Telmisartan were (1.791, 3.434min) and (10-30, 10-50), respectively.The method has been validated for linearity, accuracy and precision, robustness and limit of detection and limit of quantitation.The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.86µg/ml and 2.58µg/ml for Efonidipine and 1.28µg/ml 3.84µg/ml for Telmisartan respectively.The developed method was found to be accurate, precise and selective for simultaneous determination of Efonidipine and Telmisartan in tablets.