Supplemental data for: Assessing the accumulation of microplastics in earthworms (Eisenia fetida) using traditional bioaccumulation modeling and synchrotron-based microcomputed tomography

Raw data from study on assessing the accumulation of microplastics in earthworms (Eisenia fetida) using traditional bioaccumulation modeling and synchrotron-based microcomputed tomography. Abstract: Microplastics (MPs) are recognized as a ubiquitous contaminant in terrestrial ecosystems. Earthworms, which play key roles in soil structure, nutrient cycling, and ecosystem function, are important bioindicators for assessing MP exposure and fate. However, uncertainties remain regarding MP toxicokinetics in earthworms, including ingestion dynamics, retention, and the potential for translocation across the gut epithelium. This study investigated the movement and fate of MPs in Eisenia fetida by quantifying uptake and elimination across five common MP morphologies (films, fibres, foams, fragments, and spheres) and by using synchrotron-based microcomputed tomography (SR-µCT) to visualize internal distributions of 5-22 µm glass microspheres and 45-53 µm polyethylene microspheres. Across 21-day exposure and depuration phases, earthworms rapidly ingested all MP morphologies, reaching steady state before Day 10 for most types, and efficiently eliminated MPs once removed from spiked soils. Elimination rate constants (1.27–2.34 day⁻¹) corresponded to short half-lives (