IMPACT OF AQUA FIX ON BRAIN BIOCHEMICAL CHANGES IN AEROMONIASIS INDUCED LABEO ROHITA

The present study was conducted to evaluate the immunomodulatory effect of Aqua Fix, a herbal feed supplement on brain biochemical alterations in Labeo rohita experimentally infected with Aeromonas hydrophila. Three groups(A,B,C) of six months old fish were employed; groups A and B were treated with Aqua Fix for 4 days, on day 5 fish of group B and C were infected with Aeromonas hydrophila @ 10-6CFU/fish(fish of group C were fed with normal diet for 4 days).Controls (group D) were untreated and uninfected. Necropsies were made on day 1, 2,3,4,7 and 15 after infection in fish of group B and C; fish of group A and D were also necropsied on same designated days. Brain protein, carbohydrate, DNA and RNA were analysed following standard methods and results were subjected to statistical analysis and found that Aqua Fix modulated immunity to enhance the level of protein, carbohydrate, DNA and RNA in brain. Group C showed marked decrease of all the above biomolecules in L.rohita due to stress caused by aeromoniasis .These findings suggests the protective effects of Aqua Fix against aeromoniasis induced L.rohita.