AN EVALUATION OF PHYTOCHEMICAL, INVITRO ANTIOXIDANT AND ANTIINFLAMMATORY ACTIVITIES OF LEAF AND BARK ETHANOLIC EXTRACT OF MADHUCA LONGIFOLIA

Madhuca longifolia is commonly found in Indian subcontinent and its parts have been used traditionally in indigenous medicine due to its medicinal value. The present study is focused on the phytochemical analysis of ethanolic extract leaf and bark of M. longifolia and found the presence of carbohydrates, tannins, phenols, flavonoids, cardiac glycosides, coumarins, quinones, steroids, and phytosterols. The phytochemical phenol, flavonoids and tannins were estimated as 166.75 ± 14.60 mg GAE/g dry weight, 307.93 ± 36.33 mg quercetin equivalents per gram of dry weight and 23.81 ± 10.00 mg/g respectively. The antioxidant activity was determined by DPPH radical scavenging activity and hydrogen peroxide scavenging activity. The DPPH free radical scavenging was estimated as 71.35 ± 0.003% at 250 µg/ml. The maximum hydrogen peroxide scavenging activity was recorded at 50 µg/ml as 49.34 ± 0.007%. The anti-inflammatory activity was determined by bovine serum albumin denaturation assay, and it was found as 44.55 ± 0.011% inhibition at 100 µg/ml. The current study revealed the diverse phytochemical constitutes, antioxidant and anti-inflammatory activity of ethanolic extract of leaf and bark of M. longifolia.