Association of MMP-9 and PAD-4 according to UC extension and severity

Matrix metalloproteinase-9 (MMP-9) and Peptidyl Arginine Deiminase-4, enzymes released mainly from activated neutrophils in the colonic mucosa of patients with Ulcerative colitis (UC) and involved in intestinal barrier dysfunctions.This study aimed to determine the relation of serum and colon biopsies of MMP-9 and PAD-4 in patient with UC to access disease extension and severity.The study group comprised 60 adults newly diagnosed with UC and 20 control groups.Venous blood sample used for both MMP-9 and PAD-4 by ELISA technique while, Real-time PCR was used to study tissue expression of MMP-9 and PAD-4 in colon biopsies obtained during colonoscopy.Both serum and tissue MMP-9 and PAD-4 were significantly elevated in UC patients in compared to control group, although MMP-9 relationship to both extension and severity was significant except when compared control to proctitis and control to mild group.On the other hand, both serum and tissue expression of PAD-4 was significantly correlated to extension and severity indices with better sensitivity and specificity than MMP-9.Combining MMP-9 and PAD-4 in serum and colon biopsies obtained during colonoscopy may be valuable in improving disease severity performance.Matrix metalloproteinase-9 (MMP-9) and Peptidyl Arginine Deiminase-4, enzymes released mainly from activated neutrophils in the colonic mucosa of patients with Ulcerative colitis (UC) and involved in intestinal barrier dysfunctions.Aims: This study aimed to determine the relation of serum and colon biopsies of MMP-9 and PAD-4 in patient with UC to access disease extension and severity.The study group composed of newly diagnosed UC (60 adults) and healthy control group (20 Adults).Venous blood sample used for both MMP-9 and PAD-4 by ELISA technique while, Real-time PCR was used to study tissue expression levels of MMP-9 and PAD-4 in colon mucosal tissue biopsies obtained during colonoscopy.Results: Both serum and tissue enzymes level of MMP-9 and PAD-4 were significantly elevated on UC patients in compared to healthy control group, although MMP-9 relationship to both extension and severity was significant except when compared control to proctitis and control to mild group.On the other hand, both serum and tissue expression of PAD-4 was significantly correlated to extension and severity indices with better sensitivity and specificity than MMP-9.Combining MMP-9 and PAD-4 in serum and colon biopsies obtained during colonoscopy may be valuable in improving disease severity performance.