Integrated multiplex PCR and metatranscriptomics reveal upper-lower airway microbial landscapes in pediatric respiratory infections

ScienceDB 2026 Dataset Open access

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Authors: Yanqun Wang, Yingkang Jin, Jincun Zhao, Jingxian Zhao
DOI: 10.57760/sciencedb.36281
Cited by: 0

Abstract

Respiratory samples were collected from 514 hospitalized pediatric patients diagnosed with ARTIs. A total of 505 nasopharyngeal swabs (NS) and 85 bronchoalveolar lavage fluid (BALF) samples were obtained. NS and BALF samples were tested using a 22-target multiplex PCR panel and subsequently stratified by pathogen status for pooled metatranscriptomic sequencing to profile active microbial communities, viral genotypes, and antibiotic resistance genes.

Fields of Study & Subjects

Respiratory viral infections research Pneumonia and Respiratory Infections Microbial infections and disease research

Publication details

Journal / source
ScienceDB
Publication date
25 January 2026
Language
EN
Document type
Dataset