Analysis of Transcriptome Data from LSDV-Infected MAC-T Cells

Transcriptome sequencing was done by Megi Bio (Shanghai). Total RNA was extracted from cells using TRIzol reagent (Invitrogen) according to the manufacturer 's instructions. RNA was treated with DNase I for 30 min at 37 ° C to remove residual DNA. Qualified samples were used for library construction. Double-end sequencing libraries were prepared using the ABclonal mRNA-seq Lib Prep Kit (ABclonal, China) according to the manufacturer 's instructions. Library quality was assessed using the Agilent Bioanalyzer 4150 system. Finally, libraries were sequenced on an Illumina NovaSeq 6000 (or MGISEQ-T7) to generate 150 bp double-ended sequencing data. Bioinformatic analysis was performed using data generated from the Illumina (or BGI) platform.