The haplotype-resolved assembly of Centella asiatica and its annotation file

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Authors: 汪超
Cited by: 0

Abstract

Extract genomic DNA with CTAB method and PacBio Sequel II platform was used to construct 15 kb DNA SMRTbell library. The circular consensus sequencing (CCS) was the raw sequencing data of the library. Hi-C data was sequenced using the platform of Illumina MGISEQ-T7. SMRT Link (v9.0.0) and Fastp (v0.23.4) were used to generate CCS reads and clean Hi-C reads, respectively. Hifiasm (v0.19.9), Juicer (v2.13.07), 3d-DNA (v190716) and Juicerbox (v1.11.08) was used for genome assembly . GenomeThreader (v1.7.1), HISAT2 (v2.2.1), StringTie (v2.1.4), TransDecoder (v5.5.0), Trinity (v2.12.0), PASA (v2.5.2), Augustus (v3.3.3), and EVidenceModeler (v2.1.0) were used to predict gene structure annotation.

Fields of Study & Subjects

Genomics and Phylogenetic Studies Chromosomal and Genetic Variations Genetic Mapping and Diversity in Plants and Animals

Publication details

Journal / source
ScienceDB
Publication date
19 January 2026
Language
EN
Document type
Dataset