Effect of the Type of Cystic Ovaries on the Concentration of Some Hormones and Minerals in the Blood of Buffalo Female

This study was conducted in the laboratory of graduate studies in the Department of Animal Production, College of Agriculture, Al-Muthanna University, and the laboratories of the of Jabir Ibn Hayyan Medical University during the period 22/9/2022 to 1/10/2023. The concentrations of hormones (insulin, prolactine, estrogen and luteinizing hormone) and the elements (Ca, P, Cu and Fe) were measured in the blood of buffalo females and compared the infected and the normal females. On the other hande the immature oocytes ware subjected to the experiment for in vitro maturation (UM) using the culture medium Dulbeccos Modified Eagles Medium (DMEM) treated with the nitric oxide with two concentrations : the low concentration was (3mM) and the high concentration was (5mM), to determine the effect of the cystic ovaries (follicular and luteal) ,the presence of cumulus oophorus and the concentration of nitric oxide in the percentage of (IVM) of buffalo oocytes .The result showed a highly significant differences (P≤ 0.01) between the three groups of buffalo ovaries on the concentration of the insulin hormone in the blood, the follicular cystic ovaries recorded the highest concentration of the insulin hormone(28.61±0.15 U/mL) as compared with the luteal cystic ovaries ( 23.6 ± 0.29 U/mL ) and the normal ovaries ( 24.41± 0.14 U/mL ).