Comparative Efficacy of Microfluidics and Density Gradient Centrifugation for Sperm Preparation in IVF: A Randomized Controlled Trial

JBRA 2026 Open access

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Authors: Thuy Huong, Do Thi Minh Tam, Nguyen Thanh Hoa, Hồ Nguyệt Minh, Nguyen Manh Ha, Hung S. Ho
DOI: 10.5935/1518-0557.20250190
Cited by: 1

Abstract

OBJECTIVE: To compare the effectiveness of microfluidic sperm sorting (MC) and traditional density gradient centrifugation (DGC) in reducing sperm DNA fragmentation index (DFI) and evaluate their impact on clinical outcomes in IVF cycles. METHODS: In this randomized controlled trial, 119 couples undergoing IVF were allocated to either the MC or DGC group. Sperm DNA fragmentation was assessed before and after preparation. Primary outcomes included live birth rate and DFI reduction. Secondary outcomes were sperm quality parameters, fertilization rate, embryo development, and pregnancy outcomes. Subgroup analysis was conducted based on initial DFI levels (

Fields of Study & Subjects

Sperm and Testicular Function Reproductive Biology and Fertility Ovarian function and disorders

Publication details

Journal / source
JBRA
Publisher
GN1 Sistemas e Publicacoes Ltd.
Volume
30
Issue
2
Pages
1–8
Publication date
1 January 2026
Language
EN